Fluorimetric assay of interaction of protein with ferrofluids

Mallik, D and Mir, Aparna and Bhattacharya, S and Nayar, S (2011) Fluorimetric assay of interaction of protein with ferrofluids. Spectrochimica Acta Part A-Molecular And Biomolecular Spectroscopy , 78 (1). pp. 332-336.

Restricted to NML users only. Others may use ->



Magnetic iron oxide nanoparticles are inherently biocompatible and are amenable to post synthesis surface modification, making them excellent candidates for many important applications. If the above can be achieved in a single-step i.e., in situ synthesis and functionalization, the results are expected to be more dramatic for sensitive detection of biomolecules. For any application, it is necessary to confer a high level of binding specificity through surface chemistry, which can be introduced by using biological moieties that possess lock-and-key interactions, like those observed in antibody-antigen and enzyme-substrate recognition. In this paper, we have synthesized water based ferrofluids with serum albumin, the major protein component of blood. A series of other ferrofluids using different biocompatible polymers have also been studied with respect to their size determined by transmission electron microscopy, magnetic behavior with the aid of vibrating sample magnetometry and binding capability to bovine serum albumin by quenching of its native fluorescence. From our results, it can be inferred that binding has taken place between magnetic particles and biomolecules, the binding constants of which indirectly reveal the efficiency of the interaction. (c) 2010 Elsevier B.V. All rights reserved.

Item Type:Article
Official URL/DOI:http://apps.isiknowledge.com/full_record.do?produc...
Uncontrolled Keywords:Aqueous ferrofluids; Fluorescence; Coating; Bovine serum albumin
Divisions:Material Science and Technology
ID Code:3045
Deposited By:Sahu A K
Deposited On:17 Jun 2011 13:02
Last Modified:21 Nov 2011 17:12
Related URLs:

Repository Staff Only: item control page