Three dimensional biphasic calcium phosphate nanocomposites for load bearing bioactive bone grafts

Abstract

Mimicking matrix mediated bio-mineralization process, three dimensional-blocks of biphasic calcium phosphate (BCP, hydroxyapatite (HA) and beta-tricalcium phosphate (TCP)) nanocomposites, having three different stoichiometries have been synthesized for possible application as load bearing synthetic bone graft or scaffolds. Biphasic blocks with three weight ratios of 20:80, 25:75 and 30:70 of HA and TCP respectively have been synthesized. Detailed structural and chemical characterization of the samples revealed a strong dependence of porosity and mechanical properties on the stoichiometry of biphasic blocks. Effect of physiological medium on the microstructure and mechanical properties of the three different blocks has also been studied. Bioactivity of the BCP block, exhibiting highest compressive strength in air as well as in physiological medium, has been evaluated through adhesion, proliferation and differentiation of mesenchymal stem cells using different markers. (C) 2015 Elsevier BM. All rights reserved.